,Genome-Wide Sequencing of 41 Rice (Oryza sativa L.) Mutated Lines Reveals Diverse Mutations Induced

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Dear Editor,Fast-neutron (FN) irradiation has been used to create mutagenized collections of many plant species (Bolon et al.,2014).FNinduced mutagenesis has clear advantages:it is an efficient means of saturating the genome,and it does not involve timeconsuming plant transformation or tissue culture.In rice,most mutant collections,although highly valuable,were generated using either T-DNA insertion or transposon tagging approaches that often induce mutations unlinked to the insertion and complicating analysis (Wang et al.,2013).Another disadvantage of some of these collections is that they were created in rice varieties that are photoperiod-sensitive and often have long generation times.
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