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[目的]探讨并比较纳米Si O2(nano-Si O2)与介孔Si O2(SBA-15)对人脐静脉血管内皮细胞(HUVEC)的毒性效应及氧化损伤作用。[方法]将nano-Si O2、SBA-15按照不同作用浓度(0.00、6.25、12.50、25.00、50.00、100.00、200.00μg/m L)对HUVEC进行染毒,作用24 h后,测定HUVEC的增殖抑制情况、培养液中乳酸脱氢酶(LDH)的活性,及细胞内活性氧(ROS)的表达水平。[结果]与阴性对照组(0μg/m L)比较,nano-Si O2、SBA-15组细胞抑制率均升高(P<0.05),且具有明显的剂量-反应关系;低浓度(12.50μg/m L)时SBA-15组对细胞抑制率高于nano-Si O2组;随着染毒浓度增加,nano-Si O2组对细胞抑制率高于SBA-15组(P<0.05)。与阴性对照组比较,nano-Si O2、SBA-15组LDH、ROS水平均升高(P<0.05),且随着染毒浓度的升高呈现明显的剂量-反应关系(P<0.05);各剂量SBA-15染毒组的LDH和ROS水平均小于同剂量nano-Si O2染毒组(P<0.05)。[结论]nano-Si O2与SBA-15对血管内皮细胞均存在细胞毒性效应,氧化损伤可能是其细胞毒性机制之一。与nano-Si O2组相比,SBA-15细胞毒性较低。
[Objective] To explore and compare the toxic effects and oxidative damages of nano-Si O2 and SBA-15 on human umbilical vein endothelial cells (HUVECs). [Methods] The HUVECs were exposed to different concentrations (0.00,6.25,12.50,25.00,50.00,100.00,200.00μg / m L) of nano-Si O2 and SBA-15 for 24 h. The proliferation of HUVECs Inhibition, activity of lactate dehydrogenase (LDH) in culture broth, and expression of intracellular reactive oxygen species (ROS). [Results] Compared with the negative control group (0μg / m L), the cell inhibition rates in nano-Si O2 and SBA-15 groups were significantly increased (P <0.05), and had a dose-response relationship. / m L), the inhibitory rate of SBA-15 group was higher than that of nano-Si O2 group. With the increase of exposure concentration, the inhibition rate of nano-Si O2 group was higher than that of SBA-15 group (P <0.05). Compared with the negative control group, the levels of LDH and ROS in nano-Si O2 and SBA-15 groups were significantly increased (P <0.05), and the dose-response relationship was obvious with the increase of exposure concentration (P <0.05) The levels of LDH and ROS in SBA-15 treated groups were all less than those in the same dose of nano-Si O2 groups (P <0.05). [Conclusion] Both nano-Si O2 and SBA-15 have cytotoxic effect on vascular endothelial cells. Oxidative damage may be one of the cytotoxic mechanisms. SBA-15 cells are less cytotoxic than the nano-Si O2 group.