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目的以高滴度天然抗角蛋白自身抗体(AK auto Ab)转基因小鼠为模型,分析AK auto Ab在抵御金黄色葡萄球菌感染中的作用。方法取天然抗角蛋白自身抗体转基因小鼠及阴性对照小鼠,ELISA法检测小鼠腹腔冲洗液总IgM、抗角蛋白IgM抗体以及抗金黄色葡萄球菌IgM抗体水平:腹腔接种金黄色葡萄球菌制备感染模型,检测腹腔接种金黄色葡萄球菌后腹腔及肾脏菌落形成单位数量.测定菌负荷,流式细胞仪检测中性粒细胞比率、炎症因子浓度等。结果ELISA检测发现,转基因小鼠腹腔冲洗液抗角蛋白IgM、抗金黄色葡萄球菌IgM水平高于阴性对照小鼠.差异有统计学意义(P<0.01);菌落形成实验显示,转基因小鼠腹腔及肾脏菌负荷低于阴性对照(P<0.01):流式细胞仪分析发现.转基因小鼠腹腔中性粒细胞比率、炎症因子浓度低于阴性对照(P<0.01)。结论AK auto Ab在小鼠体内对金黄色葡萄球菌感染具有良好的保护作用。
OBJECTIVE: To analyze the role of AK auto Ab in combating S. aureus infection by using high titer natural keratin autoantibodies (AK auto Ab) transgenic mice as a model. Methods The natural anti-keratin autoantibodies transgenic mice and negative control mice were obtained. The levels of total IgM, anti-keratin IgM and anti-Staphylococcus aureus IgM antibodies in mice peritoneal irrigator were detected by ELISA: Staphylococcus aureus Infection model was established to detect the number of colony forming units in peritoneal cavity and kidney after intraperitoneal inoculation of Staphylococcus aureus, the bacterial load, the neutrophil ratio detected by flow cytometry, the concentration of inflammatory cytokines and so on. Results The results of ELISA showed that the levels of anti-keratin IgM and anti-Staphylococcus aureus IgM in the peritoneal wash fluid of transgenic mice were significantly higher than those of the negative control mice (P <0.01). The colony formation assay showed that the intraperitoneal (P <0.01). Flow cytometry analysis showed that the peritoneal neutrophil ratio and the concentration of inflammatory cytokines in transgenic mice were lower than those in the negative control (P <0.01). Conclusion AK auto Ab has a good protective effect against Staphylococcus aureus in mice.