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本文报告了人末梢血单核细胞在针对PPD的淋巴细胞增殖反应中的作用,并对人单核细胞抗原传递作用和调节作用进行了鉴别。作者从结核菌素皮试阳性的正常成人抽取静脉血,分离单个核细胞,再通过粘附的方法分离出单核细胞,剩下的淋巴细胞纯度达98％。用抗原(PPD)处理单核细胞后加入到自身的淋巴细胞中,培养后测定淋巴细胞的~3H-胸腺嘧啶核苷掺入率。结果表明抗原处理的单核细胞可以使淋巴细胞致敏。增加抗原处理的单核细胞数(直到单核细胞与淋巴细胞的比例为0.5:1),淋巴细胞的增殖反应也相应增强。这说明单核细胞的功能之一是将抗原传递给反应性淋巴细胞。作者将新鲜单核细胞加到淋巴细胞中,直接用PPD刺激,发现不同数量的单核细胞
This paper reports the role of human peripheral blood mononuclear cells in lymphocyte proliferation response to PPD and the identification of human monocyte antigen delivery and modulation. The authors extracted venous blood from normal adults tested positive for tuberculin skin, isolated mononuclear cells and isolated mononuclear cells by adherence. The purity of the remaining lymphocytes was 98%. Mononuclear cells were treated with antigens (PPD) and added to their own lymphocytes. After incubation, the 3H-thymidine incorporation of lymphocytes was determined. The results showed that antigen-treated monocytes sensitized lymphocytes. Increasing the number of antigen-treated monocytes (until the ratio of monocytes to lymphocytes was 0.5: 1), the lymphocyte proliferative response was also correspondingly increased. This shows that one of the functions of monocytes is to deliver the antigen to reactive lymphocytes. The authors added fresh monocytes to lymphocytes and stimulated them directly with PPD and found that different numbers of monocytes