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目的:探讨20(S)-人参皂苷-Rh2(GS-Rh2)对人膀胱癌T24细胞增殖和凋亡的影响及其可能的机制。方法:采用MTT法检测GS-Rh2对T24细胞增殖抑制率;光学显微镜观察GS-Rh2作用前后细胞形态变化;流式细胞仪检测GS-Rh2对T24细胞凋亡和细胞周期分布影响;RT-PCR检测mRNA表达。结果:GS-Rh2在5 mg/L～21.97mg/L浓度范围内,对T24细胞增殖无明显抑制作用;21.97 mg/L～160mg/L浓度范围内,GS-Rh2对人膀胱癌T24细胞的生长有抑制作用,呈量-效关系,IC50为37mg/L。T24细胞经GS-Rh2(40mg/L)作用后光镜下呈死亡形态改变;细胞增殖指数(PI)由42.534%下降至31.03%;早期凋亡细胞数明显增加;p53和myc mRNA表达下降。结论:GS-Rh2能显著抑制人膀胱癌T24细胞增殖、诱导T24细胞凋亡,其机制可能与GS-Rh2下调P53和myc mRNA表达,阻滞细胞进入S/G2期等有关。
Objective: To investigate the effect of 20 (S) -ginsenoside-Rh2 (GS-Rh2) on the proliferation and apoptosis of human bladder cancer T24 cells and its possible mechanism. Rhodamine T and MTT assay were used to detect the effect of GS-Rh2 on T24 cell proliferation. MTT assay was used to detect the effect of GS-Rh2 on the proliferation of T24 cells. The morphological changes of cells were observed by light microscopy before and after GS-Rh2 treatment. Detection of mRNA expression. Results: GS-Rh2 had no significant inhibitory effect on the proliferation of T24 cells in the concentration range of 5 mg / L ~ 21.97 mg / L. In the concentration range of 21.97 mg / L ~ 160 mg / L, GS- Growth inhibition, showed a dose-effect relationship, IC50 was 37mg / L. The apoptotic morphology of T24 cells was changed by GS-Rh2 (40 mg / L), the cell proliferation index (PI) decreased from 42.534% to 31.03%, the number of apoptotic cells increased significantly, and the expression of p53 and myc mRNA decreased. Conclusion: GS-Rh2 can significantly inhibit the proliferation of human bladder cancer T24 cells and induce the apoptosis of T24 cells. The mechanism may be related to the downregulation of P53 and myc mRNA by GS-Rh2 and the arrest of cells entering the S / G2 phase.