论文部分内容阅读
目的 :研究凋亡素对人成骨肉瘤细胞OS 732多药耐药株R OS 732的作用。方法 :凋亡素基因VP3 经酶切后克隆入质粒 pIRES1,获得重组质粒pIRVP3,通过脂质体法 pIRVP3转染R OS 732细胞 ,RT PCR检测凋亡素在细胞中的表达 ;光镜及电镜下观察瘤细胞 ;提取瘤细胞基因组DNA进行琼脂糖凝胶电泳 ;流式细胞仪检测凋亡率。结果 :凋亡素基因已在转录水平表达 ;光镜及电镜下 2组细胞形态变化明显 ;电泳见转染组DNA呈梯状条带 ,对照组为单一条带 ;转染组凋亡率明显高于空白对照组 (P <0 .0 1)。结论 :凋亡素可有效诱导R OS 732细胞凋亡。
Objective : To study the effect of apoptin on human OS 732 multidrug resistant strain R OS 732 of human osteosarcoma cells. METHODS: Apoptin gene VP3 was digested and cloned into plasmid pIRES1 to obtain recombinant plasmid pIRVP3. ROS 732 cells were transfected with pIRVP3 liposome and RT-PCR was used to detect the expression of apoptin in cells. Light microscope and electron microscope The tumor cells were observed; the genomic DNA of the tumor cells was extracted and subjected to agarose gel electrophoresis; and the apoptosis rate was measured by flow cytometry. RESULTS: The apoptin gene was expressed at the transcriptional level. The morphological changes of the cells in the two groups were obvious under light microscope and electron microscope. The electrophoresis showed that the DNA of the transfection group was a ladder-like band and the control group was a single band; the transfection group had a significant apoptosis rate. Higher than the blank control group (P < 0.01). Conclusion: Apoptin can effectively induce apoptosis in R OS 732 cells.