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目的探讨虾青素对H_2O_2导致的HepG2细胞线粒体氧化损伤及生存能力下降的保护作用。方法采用H_2O_2损伤HepG2细胞,测定细胞内Ca~(2+)浓度及线粒体膜电位、胞浆中细胞色素c(Cyt-c)的阳性表达率变化考察了虾青素对活性氧所致线粒体氧化损伤的保护作用;测定细胞存活率、乳酸脱氢酶(LDH)释放率考察了虾青素对活性氧所致细胞生存能力下降的保护作用。结果虾青素(1.0×10~(-7)、1.0×10~(-6)、1.0×10~(-5)mol·L~(-1))能明显抑制H_2O_2导致的细胞内Ca~(2+)浓度升高、线粒体膜电位降低、Cyt-c的释放增加;以及细胞存活率下降、LDH释放率增加。结论虾青素对H_2O_2所致细胞线粒体氧化损伤及细胞生存能力下降具有明显的保护作用,其机制可能与其抗氧化作用有关。
Objective To investigate the protective effect of astaxanthin on mitochondrial oxidative damage and viability of HepG2 cells induced by H_2O_2. Methods HepG2 cells were treated with H 2 O 2, and the changes of intracellular Ca 2+ concentration, mitochondrial membrane potential and cytosolic cytochrome c (Cyt-c) expression were examined. The effects of astaxanthin on mitochondria oxidation induced by reactive oxygen species The protective effect of astaxanthin on the viability of cells induced by reactive oxygen species (ROS) was investigated by measuring the cell viability and the rate of lactate dehydrogenase (LDH) release. Results Astaxanthin (1.0 × 10 ~ (-7), 1.0 × 10 ~ (-6), 1.0 × 10 ~ (-5) mol·L ~ (-1)) could significantly inhibit the intracellular Ca ~ (2+) concentration, mitochondrial membrane potential decreased, Cyt-c release increased; and cell viability decreased, LDH release rate increased. Conclusion Astaxanthin has a significant protective effect on mitochondrial oxidative damage and cell viability induced by H_2O_2, and its mechanism may be related to its antioxidative effect.