论文部分内容阅读
利用10对AFLP引物及13对SSR引物,分析了43份来自西藏地区部分野生类型油菜种质的遗传多样性。10对AFLP引物共得到276条清晰的谱带,其中多态性带214条,多态性位点比率为77.5%,平均每对AFLP引物得到21.4条多态性带。13对SSR引物共扩增出57条带,其中多态性带51条,多态性位点比率为89.5%,说明西藏野生油菜遗传多样性丰富。通过对西藏野生类型油菜资源的遗传距离以及聚类分析,可将西藏野生类油菜分为两大类群,分别为白菜型和芥菜型野生油菜种质资源;两种分子标记适合揭示西藏野生油菜的遗传多样性。
Using 10 pairs of AFLP primers and 13 pairs of SSR primers, we analyzed the genetic diversity of 43 wild type rapeseed germplasms from Tibet. A total of 276 clear bands were obtained from 10 pairs of AFLP primers, of which 214 were polymorphic bands with a polymorphism locus ratio of 77.5%. An average of 21.4 polymorphic bands were obtained for each pair of AFLP primers. Thirteen pairs of SSR primers amplified 57 bands, of which 51 were polymorphic, with a polymorphic locus ratio of 89.5%, indicating that the genetic diversity of Tibetan wild rape is abundant. Based on the genetic distance and cluster analysis of Tibet wild type rapeseed resources, the wild type rapeseed in Tibet can be divided into two groups, which are the germplasm resources of Brassica campestris and Brassica juncea. The two molecular markers are suitable to reveal the wild rape Genetic diversity.