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AIM:To analyze the relationship between clinical features and epithelial mesenchymal transition(EMT) in retinoblastoma(RB),further to investigate whether miR-200c regulates the EMT and migration of RB cells.METHODS: Expression of EMT-related markers and tumorrelated factors were detected by immuno-histochemistry analysis in RB tissue from 29 cases. Correlations between their expression and clinical characteristics were analyzed. The regulation effects of miR-200c on EMT-related markers,tumor-related factors were observed in m RNA level and protein level by real-time polymerase chain reaction(PCR) and Western blot,respectively,in Y79 and Weri-rb1 cells. Its effects on migration force of these RB cell lines were also detected with Transwell test.RESULTS: Lower expression of E-cadherin was present in the cases with malignant prognosis. Mi R-200c promoted the expression of E-cadherin and decreased the expression of Vimentin and N-cadherin in Y79 and Weri-rb1 cells. Migration force of RB cells could be inhibited by miR-200c.CONCLUSION: EMT might be associated with bad prognosis in RB. Mi R-200c suppresses the migration of retinoblastomatous cells by reverse EMT.
AIM: To analyze the relationship between clinical features and epithelial mesenchymal transition (EMT) in retinoblastoma (RB), further to investigate whether miR-200c regulates the EMT and migration of RB cells. METHODS: Expression of EMT-related markers and tumorrelated factors were detected by immuno-histochemistry analysis in RB tissue from 29 cases. Correlations between their expression and clinical characteristics were analyzed. The regulation effects of miR-200c on EMT-related markers, tumor-related factors were observed in m RNA level and protein levels by Its effects on migration force of these RB cell lines were also detected with Transwell test .RESULTS: Lower expression of E-cadherin was present in the cases with malignant prognosis. Mi R-200c promoted the expression of E-cadherin and decreased the expression of Vimentin and N-cadherin in Y79 and Weri-rb1 cells. Migration force of RB ce lls could be inhibited by miR-200c. CONCLUSION: EMT might be associated with bad prognosis in RB. Mi R-200c suppresses the migration of retinoblastoma cells by reverse EMT.