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A novel chemiluminescence system for the determination of bismerthiazol is first described in this paper. It is based on the chemiluminescence reaction of bismerthiazol and Ce(Ⅳ) in nitric acid solution. The emis-sion intensity could be enhanced greatly by Rhodamine B. The chemiluminescence intensity was proportional to bis-merthiazol concentration over the range 30-1000 μg/L. The detection limit was 12 μg/L (3σ) and the relative standard deviation is 2.4% for 500 μg/L of bismerthiazol (n = 11). The proposed method was successfully applied to the determination of bismerthiazol in water and in rice. The recovery was 96.4%-104.1%.