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目的:观察硫化氢(H_2S)对高糖腹膜透析液诱导大鼠腹膜结构和功能损伤的影响。方法:SD大鼠随机分为四组(对照组、单独H_2S组、模型组及治疗组)。第28天行腹膜平衡试验;取壁层腹膜组织观察腹膜结构变化,检测α平滑肌肌动蛋白(α-SMA)、胶原蛋白Ⅲ(Col-Ⅲ)和转化生长因子β1(TGF-β1)表达情况。检测大鼠腹膜间皮细胞单核细胞趋化蛋白1(MCP-1)、白细胞介素6(IL-6)、细胞间黏附分子1(ICAM-1)、α-SMA、E-钙黏素(E-cadherin)和TGF-β1 mRNA表达情况。结果:与对照组相比,模型组腹膜增厚伴血管增生、炎症细胞浸润增加,且间皮下α-SMA、Col-Ⅲ及TGF-β1表达显著增加(P<0.05);与模型组相比,治疗组腹膜形态明显改善,α-SMA、Col-Ⅲ及TGF-β1表达显著减少(P<0.05)。与对照组相比,模型组超滤量显著减少,腹膜通透性显著升高,治疗组大鼠腹膜转运功能显著改善(P<0.05)。2.5%葡萄糖透析液刺激腹膜间皮细胞12h使肌成纤维细胞标志蛋白α-SMA表达显著增加、上皮细胞标志蛋白E-cadherin表达显著减少,同时MCP-1、IL-6、ICAM-1和TGF-β1 mRNA表达显著增加(P<0.01);透析液中加入100μmol/L、300μmol/L NaHS可显著抑制上述炎症因子及纤维化因子表达(P<0.01)。结论:在腹膜透析液中加入外源性H_2S可显著改善腹膜结构及功能损伤,并可部分逆转腹膜间皮细胞转分化,进而减少炎症因子和纤维化因子合成。
Objective: To observe the effect of hydrogen sulfide (H 2 S) on peritoneal structure and function of rats induced by high glucose peritoneal dialysis fluid. Methods: SD rats were randomly divided into four groups (control group, H 2 S alone group, model group and treatment group). Peritoneal balance test was performed on the 28th day. Peritoneal structure changes were observed in the peritoneum of the parietal layer to detect the expression of α-SMA, Col-Ⅲ and TGF-β1 . The expressions of MCP-1, IL-6, ICAM-1, α-SMA, E-cadherin in rat peritoneal mesothelial cells (E-cadherin) and TGF-β1 mRNA expression. Results: Compared with the control group, the peritoneal thickening, vascular hyperplasia and infiltration of inflammatory cells in the model group were significantly increased, and the expressions of α-SMA, Col-Ⅲ and TGF-β1 were significantly increased (P <0.05) , The morphology of the peritoneum in the treatment group was significantly improved, and the expressions of α-SMA, Col-Ⅲ and TGF-β1 were significantly decreased (P <0.05). Compared with the control group, the amount of ultrafiltration in the model group decreased significantly, the peritoneal permeability increased significantly, and the peritoneal transport function in the treatment group was significantly improved (P <0.05). 2.5% glucose dialysate stimulated peritoneal mesothelial cells for 12h, significantly increased the expression of α-SMA and decreased the expression of E-cadherin, MCP-1, IL-6, ICAM-1 and TGF- (P <0.01). The addition of 100μmol / L and 300μmol / L NaHS to the dialysate significantly inhibited the expression of inflammatory factors and fibrosis factors (P <0.01). Conclusion: Addition of exogenous H_2S into peritoneal dialysis fluid can significantly improve the peritoneal structure and function, and can partially reverse the transdifferentiation of peritoneal mesothelial cells, thereby reducing the synthesis of inflammatory cytokines and fibrosis factors.