构建含SARS冠状病毒S蛋白基因高保守性C区基因的真核表达重组质粒pCDNA Sc作为DNA疫苗 ,其中C区位于SARS冠状病毒S蛋白S1亚基基因片段的 811～ 12 2 1bp区域的病毒外部羧酸端多肽肽段。将重组真核表达载体注射入实验小鼠肌肉 ,10 0 μl/只 ,疫苗滴鼻免疫组 ,每次以脂质体 -质粒 The eukaryotic expression recombinant plasmid pCDNA Sc containing the highly conserved C region of S protein of SARS coronavirus was constructed as a DNA vaccine in which the C region was located outside the virus in the 811-122bp region of the SARS-CoV S protein S1 subunit gene fragment Carboxylic acid terminal peptide peptide. The recombinant eukaryotic expression vector was injected into the muscle of the experimental mice, 10 0 μl / only, the vaccine intranasal immunization group, each with liposome - plasmid