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为了解儿童肺炎衣原体 (C.Pn)感染状况及临床特点 ,初步探讨协助临床诊断的方法 ,采用Hep-2细胞培养法对87例肺炎患儿的鼻咽分泌物进行C.Pn分离培养 ,同时用微量免疫荧光 (MIF)试验检测血清中的IgG、IgM。结果从11例患儿的鼻咽分泌物中分离出C.Pn ,阳性率12.64% ,其中3岁及以下阳性率12.2% (5/41例 ) ,3岁以上阳性率13 % (6/46例 ) ,两者差异无显著性。在这11例患儿中 ,8例检到相应的高效价抗体 (有1例未采到血清 )。提示C.Pn是儿童呼吸道病原之一 ,在婴幼儿中也很常见
To understand the status and clinical features of C. pneumoniae (C. pneumoniae) infection in children, we initially discussed the methods to assist clinical diagnosis. The nasopharyngeal secretions from 87 children with pneumonia were isolated and cultured by Hep-2 cell culture Serum IgG and IgM were detected by micro-immunofluorescence (MIF) assay. Results The positive rate of C.pn was 12.64% in nasopharyngeal secretions from 11 children, of which 12.2% (5/41) were younger than 3 years old and 13% (3/6) Cases), no significant difference between the two. In these 11 cases, 8 patients detected the corresponding high titer antibodies (1 without sera). Tip C. Pn is one of the respiratory pathogens in children and is also common in infants and young children