1988年我们从大连引进滑菇菌种试栽,由于制种期太长,致使严重污染。为解决这一同题,我们进行了液体菌种试验,结果表明,液体菌种培养时间比固体菌种缩短1/3,菌龄一致,污染少。现将制备工艺流程简报如下。(一)母种制备培养基为马铃薯20%、葡萄糖2%、麸皮(滤液)5%,琼脂2%。装入试管后高压灭 In 1988, we introduced the trial of the fungus species of fungi from Dalian, which caused serious pollution because of the long seed-planting period. In order to solve this problem, we carried out the liquid strain test, the results show that the liquid strain culture time shortened than the solid bacteria 1/3, the same bacteria age, less pollution. Now preparation process flow is as follows. (A) The mother’s preparation medium is 20% of potato, 2% of glucose, 5% of bran (filtrate) and 2% of agar. After the test tube into high pressure off