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本文作者采用人精子与去透明带金黄地鼠卵离体受精,继续培养异合卵至第一次卵裂获得人精子染色体标本的新技术,对两例在60Coγ射线辐射事故中全身受照男性照后6(7)年138个精于和6例正常男性240个精子染色体核型进行分析,直接观察健康人全身受60Coγ射线照射后,精子发生的远期效应,研究结果表明:①电离辐射对人精子的远期遗传学效应的靶细胞是生殖干细胞;②受损生殖干细胞来源的精子,其染色体畸变率显著增高,结构畸变类型主要是末端缺失,其次为易位、双着丝粒体、四射体等;③这些异常精子的受精能力没有受到可检出的影响:④Y-精子与X-精子的比例较对照组明显增高。
In this study, we used in vitro fertilization of human sperm and golden hamster de-zona pellucida to further develop a new technique to obtain the human sperm chromosome from the ectopic egg to the first cleavage. According to the analysis of karyotypes of 138 sperm cells in 6 (7) years and 240 normal sperm in 6 (7) years, we directly observed the long-term effects of spermatogenesis on 60Co γ-rays in healthy volunteers. The results showed that: ① ionizing radiation The target cells of long-term genetic effect on human sperm are germline stem cells; ② The sperm chromosomes of damaged spermatogenic stem cells have a significantly higher chromosome aberration rate, the main types of structural aberrations are terminal deletion, followed by translocation, , Quadrupole, etc .; ③ The fertility of these abnormal sperm was not detectable effect: ④ Y-sperm and X-sperm ratio was significantly higher than the control group.