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目的 :探讨凝血酶对人胚胎肾系膜细胞增殖和纤溶酶原激活物抑制物 1 (PAI 1 )表达的影响。方法 :采用噻唑蓝 (MTT)掺入法、Northern杂交和纤维蛋白平板法分别检测不同浓度凝血酶刺激下人胚胎肾小球系膜细胞增殖、PAI 1mRNA表达和PAI 1蛋白质活性的变化 ,并同时用人工水蛭素进行阻断实验。结果 :凝血酶呈浓度依赖性的方式促进人胚胎肾小球系膜细胞增殖、PAI 1mRNA表达和PAI 1蛋白质活性 ,水蛭素能特异地阻断凝血酶的作用。结论 :凝血酶可通过促进系膜细胞增殖、PAI 1的表达和提高其活性而导致肾脏损伤
Objective: To investigate the effect of thrombin on the proliferation of human embryonic mesangial cells and the expression of plasminogen activator inhibitor 1 (PAI 1). Methods: MTT assay, Northern hybridization and fibrin plate assay were used to detect the changes of glomerular mesangial cell proliferation, PAI 1 mRNA expression and PAI 1 protein activity in human embryos stimulated by different concentrations of thrombin Blocking experiment with artificial hirudin. Results: Thrombin promoted the proliferation of human glomerular mesangial cells in a concentration-dependent manner, PAI 1 mRNA expression and PAI 1 protein activity. Hirudin specifically blocked the action of thrombin. Conclusion: Thrombin can cause renal damage by promoting mesangial cell proliferation, PAI 1 expression and increasing its activity