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目的探讨一种可评价具有携氧功能的抗休克液体的实验动物模型。方法SD大鼠在麻醉状态下做35%、45%、55%和65%放血失血性休克模型,以乳酸林格液(LR)、全血进行复苏,观察其对失血性休克大鼠平均动脉血压(mean arterial blood pressure,MAP)、左心室收缩压(left intraventricular systolic pressure,LVSP)、左心室压力上升或下降的最大速率(the maximal change rate of left intraventricular pressure,±dp/dtmax)的影响,同时观察血气指标变化和存活率的变化。结果35%失血性休克后,输注LR和全血均可使MAP恢复到90~100mmHg水平,同时LR和全血对恢复休克后动物血流动力学指标、血气指标以及对存活率的影响无显著性差异;45%和55%失血性休克后,单纯LR输注不能使MAP恢复到90~100mmHg水平,且对休克动物的血流动力学指标和血气指标以及存活率的影响明显不及输注全血以后效果好,在55%失血性休克时差异更大。65%放血后动物很快死亡,休克程度太重,不能作为评价模型。结论55%失血性休克大鼠可作为评价具有携氧液体抗休克作用的实验动物模型。
OBJECTIVE: To study an animal model of anti-shock liquid with oxygen-carrying function. Methods Sprague-Dawley rats were anesthetized with 35%, 45%, 55% and 65% hemorrhagic shock models and resuscitated with lactated Ringer’s solution (LR) and whole blood. The effects of hemorrhagic shock on mean arterial pressure The effects of mean arterial blood pressure (MAP), left intraventricular systolic pressure (LVSP), the maximal change rate of left intraventricular pressure (± dp / dtmax) At the same time observe changes in blood gas and survival changes. Results After 35% hemorrhagic shock, MAP and MAP were recovered to 90 ~ 100mmHg after LR and whole blood injection, respectively. Meanwhile, the effects of LR and whole blood on hemodynamics, blood gas index and survival rate after resuscitation Significant difference; 45% and 55% after hemorrhagic shock, LR alone can not make the MAP returned to 90 ~ 100mmHg level, and the impact of hemodynamic parameters and blood gas indicators and survival rate of shock animals was significantly less than infusion After whole blood effect is good, greater difference in 55% hemorrhagic shock. 65% excreted animals quickly died, the degree of shock is too heavy, can not be used as evaluation model. Conclusion 55% of rats with hemorrhagic shock can be used as experimental animal models for evaluating the anti-shock effect of oxygen-carrying liquid.