应用原位分子杂交技术，检测神经生长因子（ＮＧＦ）在人骨髓巨核细胞分化发育过程中的基因表达情况，以探讨ＮＧＦ对骨髓造血组织的功能意义。实验结果表明，βＮＧＦｃＤＮＡ探针与其互补的ｍＲＮＡ杂交反应信号存在于原巨核细胞、幼巨核细胞、颗粒型巨核细胞、成熟型巨核细胞等各级不同发育阶段的巨核细胞胞浆中。实验结果提示，ＮＧＦ在骨髓巨核细胞系的发育成熟过程中可能发挥调节作用 In situ hybridization was used to detect the gene expression of nerve growth factor (NGF) during the differentiation and development of human bone marrow megakaryocytes in order to explore the functional significance of NGF on bone marrow hematopoietic tissue. The experimental results show that the signal of hybridization between βNGF cDNA probe and its complementary mRNA exists in the cytoplasm of megakaryocytes in different stages of megakaryocyte, juvenile megakaryocyte, megakaryocyte, mature megakaryocyte. The experimental results suggest that NGF may play a regulatory role in the development of bone marrow megakaryocyte cell line