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目的:研究小檗胺(Ber)对氯化钾、NE及咖啡因引起大鼠培养心肌细胞[Ca~(2+)]_i动员的影响,方法:Fluo 3-AM负载后,共聚焦法测定心肌细胞[Ca~(2+)]_i荧光强度的变化。结果:Ber对心肌细胞静息[Ca~(2+)]_i水平无影响,但可剂量依赖性地抑制KCl60mmol·L~(-1)及NE 30 μmol·L~(-1)引起的内钙动员(P<0.01),此作用与维拉帕米相似.Egtazic acid3 mmol·L~(-1)并不能增强Ber对NE引起的[Ca~(2+)]_i升高的抑制作用,无外钙时,咖啡因80-160μmol·L~(-1)的[Ca~(2+)]_i动员不受Ber的影响(P>0.05),结论:Ber与维拉帕米相似,对大鼠心肌细胞靠电压依赖性和受体操纵性钙通道而升高的胞[Ca~(2+)]_i有拮抗作用,并不影响[Ca~(2+)]_i释放。
AIM: To investigate the effect of berbamine on [Ca ~ (2 +)] _ i mobilization in cultured cardiomyocytes induced by potassium chloride, NE and caffeine.Methods: Fluo 3-AM was loaded and assayed by confocal method Changes of [Ca ~ (2 +)] _i fluorescence intensity in cardiomyocytes. RESULTS: Ber had no effect on resting [Ca ~ (2 +)] _i in cardiomyocytes, but inhibited KCl by 60 mmol·L ~ (-1) and NE 30 μmol·L ~ (-1) in a dose-dependent manner (P <0.01), which is similar to that of verapamil.Egtazic acid 3 mmol·L -1 did not enhance the inhibitory effect of Ber on NE-induced [Ca 2+] i increase, In the absence of external calcium, mobilization of Ca ~ (2 +)] _1 with 80-160 μmol·L -1 caffeine was unaffected by Ber (P> 0.05). Conclusion: Ber is similar to verapamil, The rat cardiomyocytes had antagonistic effect on cell [Ca ~ (2 +)] _ i which was increased by voltage-dependent and receptor-operated calcium channel, but did not affect the release of [Ca ~ (2 +)] _i.