目的分析检测密闭环境中悬浮颗粒物上附着微生物的种类及数量 ,为航天器舱内大气污染的控制和监测提供参考依据。方法依据U .S .AEPAIP 1 0方法 ,在密闭环境中用大流量冲击式采样器分 3级连续采样 ,颗粒物的空气动力学直径 (AD)分别为 :1 0 0 μm≥AD >1 0 μm、1 0 μm≥AD >2 μm及AD≤ 2 μm。选择室外较洁净空气以同样方法采样作为对照。对采集的颗粒物精密称量。分别培养颗粒物上的吸附微生物 ,并进行分离鉴定和分类计数。结果计算出密闭环境中总悬浮颗粒物 (TSP)、可吸入颗粒物 (IP)的质量浓度。检测出密闭环境中悬浮颗粒物上附着的微生物的种类包括 :细菌 6个种类、放线菌 2个属、真菌 6个属。结论密闭环境中的悬浮颗粒物对人体健康和仪器设备可能产生一定影响。 Objective To analyze and detect the types and quantities of microorganisms attached to suspended particulates in a closed environment to provide a reference for the control and monitoring of air pollution in spacecraft. Methods Based on the U.S.SEPAIP 10 method, a large flow impact sampler was used to continuously sample in 3 stages in an enclosed environment. The aerodynamic diameters (AD) of the particles were as follows: 1 0 0 μm ≧ AD> 1 0 μm , 10 μm ≧ AD> 2 μm and AD ≦ 2 μm. Select outdoor cleaner air sampling in the same way as a control. The collected particles were weighed accurately. Microparticles were cultured on the adsorption of microorganisms, and identification of the separation and classification. Results The mass concentrations of total suspended particulates (TSP) and respirable particulate matter (IP) were calculated in a confined environment. Detected in a closed environment on the suspended particles attached to the species of microorganisms include: 6 species of bacteria, actinomycetes 2 genera and fungi 6 genera. Conclusion Suspended particulate matter in a closed environment may have some impact on human health and equipment.