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目的 :探讨心理应激增加对感染的易感性的细胞和分子免疫学机理。方法 :采用双荧光染色流式细胞分析法对 2 0名健康大学生志愿者 (男女各半 )在应激前后进行外周血淋巴细胞免疫表型及T细胞体外丝裂原刺激早期活化抗原表达分析。连续一周期末考试被设定为心理应激。结果 :免疫表型分析显示 ,应激前后CD2、CD3、CD4、CD8、CD19、CD2 0、CD16、CD5 6等淋巴细胞的表面分子阳性的细胞的百分比的差异无统计学显著性 ;与应激前的结果相比 ,应激后的T细胞在体外培养条件下多克隆刺激剂活化后CD6 9的表达明显降低 ,植物血凝素 (phytohemagglutinin ,PHA)组CD6 9+ CD3+ /CD3+ 的百分率由应激前的 2 8.1± 4.1降低到应激后的 17.6± 3.8,佛波醇酯 (phorbol 12 ,13-dibutyrate ,PDB)组CD6 9+ CD3+ /CD3+ 的百分率由应激前的 80 .7± 6 .8降至应激后的 6 5 8± 7.9,而在没有刺激剂作用的条件下 ,T细胞CD6 9表达率应激前后的差别无显著。结论 :应激对免疫系统的影响并不在于改变外周血淋巴细胞各亚群的比例的层面上 ;心理应激能降低健康人T细胞体外活化的反应性 ,这可能与心理应激个体对感染的易感性增加有关。
OBJECTIVE: To investigate the cellular and molecular immunological mechanism of increased susceptibility to infection by psychological stress. Methods: Immunofluorescence of peripheral blood lymphocytes and early activation antigen expression of T cells stimulated by mitogen in vitro were analyzed before and after stress by using double fluorescence staining flow cytometry. The exam at the end of one consecutive week is set as psychological stress. Results: The immunophenotypic analysis showed that there was no significant difference in the percentage of cells with positive surface molecules of lymphocytes such as CD2, CD3, CD4, CD8, CD19, CD20, CD16 and CD56 before and after stress; Compared with the pre-stressed T cells, the expression of CD6 9 was significantly decreased in activated T cells stimulated with polyclonal stimuli in vitro. The percentage of CD6 9 + CD3 + / CD3 + in phytohemagglutinin (PHA) The percentage of CD6 9 + CD3 + / CD3 + in phorbol 12 (13-dibutyrate, PDB) group decreased from 80.1 ± 4.1 before stress to 17.6 ± 3.8 after stress by 80.7 ± 6 .8 dropped to 65.8 ± 7.9 after stress, while in the absence of stimulant, there was no significant difference in the expression of CD6 9 before and after stress. Conclusion: The effect of stress on the immune system does not lie in changing the proportion of peripheral blood lymphocyte subsets. Psychological stress can reduce the activation of healthy human T cells in vitro activation, which may be related to psychological stress individual infection Increased susceptibility.