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目的:研究人工牛黄对致痫大鼠行为的影响,海马及门区神经元丢失情况和谷氨酸脱羧酶(GAD)免疫反应阳性细胞数目的改变,探讨该药的抗惊厥作用。方法:采用戊四唑急性惊厥模型,参照Rac ine标准观察行为学表现,用N issl染色作海马及门区神经元计数,用免疫组织化学方法作GAD免疫反应阳性细胞计数。结果:牛黄防治组惊厥发作的潜伏时间较对照组长,发作次数较惊厥组少,而在海马及门区的神经元和在海马的GAD阳性细胞的丢失数量较惊厥组的少。结论:人工牛黄能延长惊厥发作的潜伏期,减少发作次数,减轻神经元丢失和保护GAD免疫反应阳性细胞。
OBJECTIVE: To study the effect of artificial bezoar on the behavior of epilepsy rats, the loss of neurons in the hippocampus and portal area, and the change of the number of glutamic acid decarboxylase (GAD) immunoreactive positive cells, and to investigate the anticonvulsant effects of this drug. METHODS: The pentylenetetrazole acute convulsive model was used to observe the behavioral characteristics with reference to Rac ine criteria. Nissl staining was used to count hippocampal and portal neurons. Immunohistochemistry was used to count GAD immunoreactive cells. RESULTS: The latency of convulsive seizures in the bezoar prevention group was longer than that in the control group, and the seizure frequency was less than that in the seizure group. However, the number of GAD-positive cells in the hippocampus and the hippocampus was less than that in the seizure group. Conclusion: Artificial bezoar can prolong the latency of convulsive seizures, reduce the number of seizures, reduce neuronal loss and protect GAD immunoreactive positive cells.