论文部分内容阅读
目的探索喷他佐辛对高糖环境下体外培养大鼠视网膜Müller细胞的保护作用及其作用机制。设计实验研究。研究对象体外培养的SD大鼠视网膜Müller细胞。方法体外培养的SD大鼠视网膜Müller细胞分三组:正常对照组(葡萄糖5mmol/L),高糖组(葡萄糖30 mmol/L),高糖+喷他佐辛组(葡萄糖30 mmol/L+喷他佐辛3 umol/L),分别在培养6、12、24、72小时以MTT法检测细胞活性;RT-PCR法检测Müller细胞血管内皮生长因子(vascular endothelial growth factor,VEGF)和转录激活因子4(activating transcription factor 4,ATF4)mRNA的表达;蛋白印迹法检测VEGF和ATF4蛋白的表达。主要指标细胞活性,VEGF和ATF4 mRNA和蛋白的表达。结果高糖作用72小时,高糖组与对照组相比细胞活性下降(23.3±0.1)%(P<0.05),喷他佐辛处理后可抑制由于高糖作用造成的Müller细胞活性降低,且与对照组无统计学差异。与对照组相比,高糖组VEGF、ATF4mRNA和蛋白表达量均显著增加(P<0.05),喷他佐辛处理后可降低VEGF、ATF4 mRNA和蛋白的表达与对照组无显著性差异(P>0.05)。结论喷他佐辛可提高高糖作用下体外培养视网膜Müller细胞的活性,减少高糖作用下视网膜Müller细胞VEGF、ATF4 mRNA和蛋白的表达,具有神经保护作用。
Objective To explore the protective effect and mechanism of pentazocine on cultured retinal Müller cells cultured in vitro in high glucose. Design experiment research. Research object SD rat retinal Müller cells cultured in vitro. Methods SD rat retinal Müller cells cultured in vitro were divided into three groups: normal control group (glucose 5 mmol / L), high glucose group (glucose 30 mmol / L), high glucose + pentazocine group (glucose 30 mmol / L + He and Zosin 3 umol / L). The cell viability was detected by MTT assay at 6, 12, 24 and 72 hours respectively. The expressions of vascular endothelial growth factor (VEGF) and transcriptional activator 4 (ATF4) mRNA expression; Western blotting detection of VEGF and ATF4 protein expression. The main indicators of cell activity, VEGF and ATF4 mRNA and protein expression. Results Compared with the control group, the cell viability was decreased by 23.3 ± 0.1% (P <0.05) at 72 hours in high glucose group. Pentazocine treatment inhibited the decrease of Müller cell activity caused by high glucose and No significant difference with the control group. Compared with the control group, the expression of VEGF, ATF4 mRNA and protein in high glucose group were significantly increased (P <0.05), and pentatrazin treatment could reduce the expression of VEGF, ATF4 mRNA and protein compared with the control group (P > 0.05). Conclusions Pentazocine can enhance the activity of cultured retinal Müller cells under high glucose and decrease the expression of VEGF and ATF4 mRNA and protein in retinal Müller cells under high glucose, which has the neuroprotective effect.