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以颠茄种子萌发的无菌苗为试材,采用组织培养方法,以MS为基本培养基,研究IAA、NAA、6-BA、KT对愈伤组织诱导及分化的影响,探索适宜颠茄愈伤组织诱导和分化的培养基。结果表明:在MS+KT 0.5mg/L+6-BA 1.0mg/L和MS+KT 1.0mg/L+6-BA 0.5mg/L培养基上,愈伤诱导率较高达100%,KT、6-BA添加生长素配比诱导愈伤组织效果不佳。KT单独诱导的愈伤组织宜在20d后转接,KT和6-BA配合使用诱导出的愈伤组织宜在40d后转接。生根适宜培养基为1/2MS+NAA 0.2~0.4mg/L。该研究建立了颠茄的植株再生体系,为颠茄的组织培养提供了技术体系。
Aseptic seedlings germinated from Belladonna davidiana seeds were used as materials to study the effects of IAA, NAA, 6-BA and KT on the induction and differentiation of callus using tissue culture method and MS as the basic culture medium. Wound tissue induction and differentiation medium. The results showed that the callus induction rate was as high as 100% on MS + KT 0.5mg / L + 6-BA 1.0mg / L and MS + KT 1.0mg / L + 6-BA addition of auxin ratio callus induction ineffective. KT callus induction should be transferred after 20d, KT and 6-BA induced callus should be transferred after 40d. Suitable rooting medium is 1 / 2MS + NAA 0.2 ~ 0.4mg / L. The study established a plant regeneration system belladonna, belladonna tissue culture provides a technical system.