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本文选择实验室饲养的巴拉巴按蚊玻璃市型和大劣按蚊曼谷株为材料,用薄膜电泳法测定其酯酶图式,以了解能否用生物化学的指标对两种按蚊进行鉴别。测定方法,以琼脂0.75g和聚乙烯吡咯烷酮2g加于100ml磷酸钾缓冲液中(pH6.8)溶解为介质分离酯酶,用10ml琼脂凝胶溶液制成0.1cm厚的13×15cm~2薄板。用日龄5天未吸过血成蚊,取单体标本放在一滴去离子蒸馏水中磨碎,将此液吸入滤纸片(1×5mm~2)置琼脂板上30分钟。移去纸片,用滤纸搭桥使与电极槽内磷酸缓冲液连接,在5℃冰箱中电泳90分钟,维持电流在30mA与电压300V。电泳后,用1%α和β醋酸萘酯丙酮液喷涂琼脂板,于室温中温育30分
In this paper, laboratory-based culture of Anopheles balaba and Anopheles anopheles Bangkok strains were used as materials to determine their esterase patterns by thin-film electrophoresis in order to find out whether biochemical indexes could be used to distinguish the two species of Anopheles . Assay method, agar 0.75g and polyvinylpyrrolidone 2g plus 100ml potassium phosphate buffer (pH6.8) was dissolved as a medium for esterase, 10ml agar gel solution was made of 0.1cm thick 13 × 15cm ~ 2 sheet . With blood 5 days of age did not suck blood adult mosquitoes, monomeric specimens were taken in a drop of deionized distilled water, the liquid inhalation filter paper (1 × 5mm ~ 2) set agar 30 minutes. Remove the paper, with filter paper bypass bridge with the phosphate buffer solution connected to the electrode, electrophoresis in the refrigerator at 5 ℃ 90 minutes to maintain the current at 30mA and voltage 300V. After electrophoresis, agar plates were sprayed with 1% α and β naphthyl acetate acetone solution and incubated for 30 minutes at room temperature