目的:建立用高效液相色谱法同时测定中药瓦松中槲皮素和山萘素的方法。方法:采用Thermo C18(4.6 mm×250 mm,5μm)色谱柱,流动相甲醇-醋酸盐缓冲液(53∶47),体积流量1.0 mL·min-1,柱温30℃,检测波长360 nm,进样体积为20μL。结果:槲皮素和山萘素分别在3.92~11.76 mg·L-1(r=0.999 8),10.03~30.09 mg·L-1(r=0.999 9)线性关系良好。平均回收率分别为99.85%,100.07%;RSD(n=9)分别为0.78%,1.05%。结论:该方法简便快速,结果准确,重复性好,可用于瓦松药材的质量控制。 Objective: To establish a method for the simultaneous determination of quercetin and kaempferide in Chinese traditional medicine Huasong by high performance liquid chromatography. METHODS: The mobile phase consisted of methanol-acetate buffer (53:47), volume flow rate 1.0 mL · min-1 and column temperature 30 ℃ with a wavelength of 360 nm on a Thermo C18 column (4.6 mm × 250 mm, 5 μm) , Injection volume of 20μL. Results: The linear relationship between quercetin and kaempferol were 3.92 ~ 11.76 mg · L-1 (r = 0.999 8) and 10.03 ~ 30.09 mg · L-1 (r = 0.999 9) respectively. The average recoveries were 99.85% and 100.07%, respectively. RSDs (n = 9) were 0.78% and 1.05%, respectively. Conclusion: The method is simple, rapid, accurate, reproducible and can be used for the quality control of varnishes medicinal herbs.