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目的:观察蝙蝠葛酚性碱对小鼠脑缺血再灌注后软脑膜微循环障碍的改善作用。方法:将60只小鼠随机分为假手术组、缺血再灌注组、蝙蝠葛酚性碱低、中、高剂量组、阳性对照组。用动脉夹夹闭小鼠双侧颈总动脉10 min后去掉动脉夹,制作脑缺血再灌注损伤模型,应用BI2000微循环图像处理系统,通过开放式颅窗观察各组小鼠软脑膜微静脉、微动脉血流速度和微小血管开放数目,以及蝙蝠葛酚性碱对小鼠脑缺血再灌注后软脑膜微循环障碍的改善作用。结果:缺血损伤组软脑膜微小动脉、静脉血管直径明显缩小,血流速度缓慢,与正常对照组比较,差异有显著意义(P<0.05);蝙蝠葛酚性碱可剂量依赖性增加缺血再灌注所致的软脑膜微小动、静脉血流速度和血管开放数目。结论:蝙蝠葛酚性碱可增加小鼠脑缺血再灌注脑软膜血流量,改善脑缺血后脑软膜微循环障碍,发挥对脑缺血的保护作用。
OBJECTIVE: To observe the effects of PAMD on the microcirculation disturbance of cerebral pia mater after cerebral ischemia-reperfusion in mice. Methods: Sixty mice were randomly divided into sham-operated group, ischemia-reperfusion group, low-dose, middle-dose and high-dose group, The artery clips were used to clamp the bilateral common carotid arteries for 10 min, the arterial clips were removed, and the model of cerebral ischemia-reperfusion injury was made. BI2000 microcirculation image processing system was used to observe the micro-venules , Micro arterial blood flow velocity and the number of microvessel opening, and the improvement effect of PAM on microcephalic microcirculation after cerebral ischemia-reperfusion in mice. Results: Compared with the normal control group, the diameter of venules of arterioles and venules in ischemic injury group was significantly reduced and the blood flow velocity was slowed down (P <0.05). Reperfusion-induced pia mater micro-venous flow velocity and the number of open vessels. CONCLUSION: DBP can increase the cerebral soft membrane blood flow in mice with cerebral ischemia and reperfusion, improve cerebral soft membrane microcirculation barrier after cerebral ischemia, and play a protective role in cerebral ischemia.