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从PBCV 1感染小球藻NC6 4A的细胞裂解液中提取了Lysin的粗制剂 ,酶活底物范围分析表明 ,几丁质酶、壳聚糖酶和 β 1 ,3 葡萄糖苷酶是Lysin活性的主要组成部分 ,并与小球藻细胞壁的组成成分相吻合。其中几丁酯酶和壳聚糖酶 ,特别是几丁酯酶在裂解小球藻细胞壁的过程中发挥了重要的作用。Lysin粗制剂经FPLC分离纯化得到分子量分别为 5 2kD、5 6kD的两个几丁质酶 (Chil和Chi2 )和一个分子量为 3 6kD的壳聚糖酶
Crude Lysin was extracted from cell lysate of PBCV1-infected chlorella NC6 4A. The range of enzyme substrate showed that chitinase, chitosanase and β1, 3 glucosidase were Lysin activity The main components, and chlorella cell wall composition consistent. Chitin esterase and chitosanase, especially chitin esterase, play an important role in cleaving the cell wall of Chlorella. Lysin crude was separated and purified by FPLC to obtain two chitinase (Chil and Chi2) with a molecular weight of 52kD and 56kD and a chitosanase with a molecular weight of 36kD