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建立人源蓝氏贾第鞭毛虫C57BL/ 6N小鼠动物感染模型。方法 用分离自河北和江苏两地蓝氏贾第鞭毛虫感染者的包囊 (河北株 ,CD和徐州株 ,XZ)分别感染两组C57BL/6N小鼠 ,收集受染鼠粪便 ,用蔗糖梯度离心法分离纯化包囊 ,计算排囊数量 ,分析排囊规律 ;观察小肠病理组织学改变。结果 C57BL/ 6N小鼠对此 2株蓝氏贾第鞭毛虫具有很高的易感性 ,接受具有活力的 1× 1 0 4 个包囊 /只的 3 6只小鼠 ,均获得感染 ,其中呈间歇性排囊者为 3 2只 ( 88 9% ) ,连续性排囊者 4只 ( 1 1 1 % )。排囊潜伏期为接种后第3d ,高峰期为第 6d。 2小时所排粪便含包囊数最高可达 2 3× 1 0 7个 /g粪。小肠粘膜出现表面分泌物增加 ,间质充血、水肿 ,炎性细胞浸润 ,粘膜坏死脱落等病理变化。结论 C57BL/ 6N小鼠可作为建立蓝氏贾第鞭毛虫感染的良好动物模型
Establishment of an animal model of infection with Giardia lamblia C57BL / 6N mice. Methods Two groups of C57BL / 6N mice were infected by cysts (Hebei strain, CD strain and Xuzhou strain, XZ strain) respectively from Hebei and Jiangsu Provinces. The feces of the infected mice were collected and stained with sucrose gradient Centrifugal separation and purification of cysts, calculation of the number of bags, analysis of the rules of the bowel; observed histopathological changes in the small intestine. Results C57BL / 6N mice had high susceptibility to the two Giardia lamblia, and all of 36 mice with viable 1 × 104 cysts per mouse were infected with There were 32 (88.9%) patients with intermittent drainage and 4 (11.1%) with continuous drainage. Drainage latency was 3d after inoculation, peaked at 6d. 2 hours excretion of the number of cysts containing up to 2 3 × 107 / g manure. Small intestinal mucosa increased surface secretion, interstitial congestion, edema, inflammatory cell infiltration, mucosal necrosis and other pathological changes. Conclusion C57BL / 6N mice can be used as a good animal model to establish Giardia lamblia infection