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目的探讨赤芍总苷对非小细胞肺癌模型大鼠MDR1-mRNA、MRP-mRNA及LRP-mRNA基因表达影响效果。方法取50只雄性大鼠随机分为正常组5只,给予0.9%氯化钠;剩下45只大鼠分别构建鳞状细胞癌、腺癌、大细胞癌肺癌模型各15只,各模型构建成功后分别分为模型组、赤芍总苷组和环磷酰胺组,每组各5只。模型组背静脉注射0.9%氯化钠1 m L;赤芍总苷组每天背静脉注射赤芍总苷240 mg·kg-1;环磷酰胺组每天背静脉注射环磷酰胺0.4 m L·kg-1,各组均连续治疗7 d。试验结束后,处死各组大鼠,并取尾静脉血及肺组织,进行肿瘤标记物、白细胞水平检测,制备肺组织切片,并通过聚合酶链反应法检测MDR1-mRNA、MRP-mRNA及LRP-mRNA基因表达水平。结果赤芍总苷组和环磷酰胺组大鼠与模型组相比,癌胚抗原、糖蛋白抗原50水平、白细胞水平和白细胞最低值均有不同程度降低,其中赤芍总苷组癌胚抗原、糖蛋白抗原50水平显著低于环磷酰胺组(P<0.05),而赤芍总苷组白细胞和白细胞最低值较环磷酰胺组明显增高(P<0.05)。赤芍总苷组MDR1基因、MRP基因和LRP基因较环磷酰胺组表达量均明显增加(P<0.05)。结论赤芍总苷能够明显抑制肿瘤细胞,并降低肺耐药蛋白及多药耐药基因的表达,从而提高机体对药物的耐受性和敏感性。
Objective To investigate the effect of total paeony glycoside on MDR1-mRNA, MRP-mRNA and LRP-mRNA gene expression in non-small cell lung cancer model rats. Methods Fifty male rats were randomly divided into normal group (n = 5) and 0.9% sodium chloride. The remaining 45 rats were screened for squamous cell carcinoma, adenocarcinoma and large cell carcinoma respectively. After the success were divided into model group, total glycosides group and cyclophosphamide group, each group of five. In the model group, 0.9% sodium chloride (1 mL) was injected intravenously into the venous blood of patients in the model group. Radix phlorizinoside group (240 mg · kg -1) -1, each group were treated for 7 days. At the end of the experiment, the rats in each group were sacrificed and the tail vein and lung tissue were taken for detection of tumor markers and leukocytes. Lung tissue sections were prepared and the expression of MDR1-mRNA, MRP-mRNA and LRP were detected by polymerase chain reaction -mRNA gene expression level. Results Compared with the model group, the levels of 50, 22 and 40 of leukocyte and leucocyte in the groups of TPA and cyclophosphamide were all decreased to some extent. Among them, (P <0.05). However, the lowest level of white blood cells and white blood cells in GPK group was significantly higher than that of cyclophosphamide group (P <0.05). The expression of MDR1 gene, MRP gene and LRP gene in erythrocyte glycoside group were significantly increased compared with cyclophosphamide group (P <0.05). Conclusion TPG can significantly inhibit tumor cells, and reduce the expression of lung resistance protein and multidrug resistance genes, thereby enhancing the body’s tolerance to drugs and sensitivity.