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目的:一氧化氮(NO)是阴茎勃起的关键因子,其生成主要由一氧化氮合酶(NOS)调节,而磷酸化Erk1/2(P-Erk1/2)和磷酸化Akt1(P-Akt1)均能调节一氧化氮合酶(NOS)的表达及活性从而影响阴茎勃起。本实验研究P-Erk1/2和P-Akt1激酶在老年大鼠阴茎海绵体中的表达,探讨其在老年大鼠勃起功能障碍(ED)发生中的可能作用。方法:A组(2月龄)和B组(18月龄)雄性SD大鼠各10只,测其血清睾酮(T),免疫组化和RT-PCR方法检测大鼠阴茎海绵体中P-Erk1/2和P-Akt1的表达水平。结果:血清T值在B组[(4.73±0.94)nmol/L]较A组[(9.57±1.57)nmol/L]显著下降(P<0.05)。P-Erk1、P-Erk2的mRNA和P-Erk1/2蛋白的相对表达量(积分光密度值IA)在B组(0.95±0.06、0.92±0.05、32.09±8.45)较A组(0.47±0.09、0.61±0.11、7.50±1.81)显著升高(P<0.05);P-Akt1的mRNA和P-Akt1蛋白的相对表达量在B组(0.94±0.05、10.93±3.06)与A组(0.97±0.04、11.67±5.61)无显著差异(P>0.05)。结论:P-Erk1/2的过表达可能是老年性ED发生的机制之一。
AIM: Nitric oxide (NO) is a key factor in penile erection and its production is mainly regulated by nitric oxide synthase (NOS). Phosphorylation of Erk1 / 2 (P-Erk1 / 2) and phosphorylation of Akt1 ) Can regulate nitric oxide synthase (NOS) expression and activity thus affecting penile erection. This study was designed to investigate the possible role of P-Erk1 / 2 and P-Akt1 kinases in the development of erectile dysfunction (ED) in the aged rat corpus cavernosum. Methods: Ten male Sprague-Dawley rats of group A (2 months old) and group B (18 months old) were randomly divided into three groups: testosterone (T), immunohistochemistry and RT-PCR. Erk1 / 2 and P-Akt1 expression levels. Results: The serum T value in group B was significantly lower than that in group A [(4.73 ± 0.94) nmol / L vs [9.57 ± 1.57] nmol / L] (P <0.05). The relative expression of P-Erk1 and P-Erk2 mRNA and P-Erk1 / 2 protein in group B (0.95 ± 0.06,0.92 ± 0.05,32.09 ± 8.45) was significantly higher than that in group A (0.47 ± 0.09 , 0.61 ± 0.11 and 7.50 ± 1.81 respectively) (P <0.05). The relative expression of P-Akt1 mRNA and P-Akt1 in group B (0.94 ± 0.05,10.93 ± 3.06) and group A 0.04, 11.67 ± 5.61) no significant difference (P> 0.05). Conclusion: Overexpression of P-Erk1 / 2 may be one of the mechanisms of senile ED.