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作者研究了自胰岛素生产过程中分离纯化胰高血糖素的方法。以胰岛素盐析物为起始物,经大孔强酸离子交换树脂柱层析,胰岛素不被吸附,随流出液流出(A),以NH_4OH稀溶液洗脱获得含有胰高血糖素的蛋白质溶液(B)。B液经凝胶过滤法分离,峰Ⅲ为富含胰高血糖素部分(C)。C液经DEAE—纤维素柱层析分离,其峰Ⅱ为电泳纯胰高血糖素(D)。标准品与D样品电泳行为相一致。兔血糖检查证明D保留有全部活性。以D浓缩物在pH 8.5下结晶,获得菱形十二面体结晶。以A制备胰岛素也同时获得胰岛素结晶。以上经离子交换树脂、凝胶过滤及DEAE—纤维素柱层析所得样品均经一种新型吸附树脂脱盐、浓缩。胰高血糖素收率为1 mg/kg猪胰。为自胰岛素生产过程中同时获得胰岛素及胰高血糖素提供了新的方法。
The authors studied methods for the isolation and purification of glucagon from the production of insulin. The insulin salt solution was used as the starting material, and the insulin was not adsorbed by the column chromatography of the macroporous strong acid ion exchange resin. The eluted effluent (A) was eluted with a dilute NH 4 OH solution to obtain the glucagon-containing protein solution ( B). Solution B was separated by gel filtration and peak III was a glucagon-rich fraction (C). The liquid C was separated by DEAE-cellulose column chromatography and its peak II was electrophoretically pure glucagon (D). The standard product is consistent with the electrophoretic behavior of the D sample. A rabbit blood glucose test demonstrated that D retained all activity. The D concentrate was crystallized at pH 8.5 to obtain diamond-shaped dodecahedral crystals. Insulin production from A also yields insulin crystals. The above samples obtained by ion exchange resin, gel filtration and DEAE-cellulose column chromatography were all desalted and concentrated by a novel adsorption resin. The glucagon yield was 1 mg/kg porcine pancreas. Provides a new way to obtain insulin and glucagon simultaneously from insulin production.