质谱已成为肽和蛋白分析的重要工具 ,主要归功于一些软电离技术的应用 ,如电喷雾 (ＥＳＩ)和基体辅助激光解吸电离 (ＭＡＬＤＩ)。生物大分子多为极性、难挥发化合物 ,不易气化 ,用传统质谱无法测定。但随着新的离子化技术的广泛应用 ,现已能高效地电离一些完整或片断的大分子 Mass spectrometry has become an important tool for peptide and protein analysis due to several applications of soft ionization techniques such as electrospray (ESI) and matrix-assisted laser desorption ionization (MALDI). Biological macromolecules are mostly polar, less volatile compounds, not easy to gasification, with conventional mass spectrometry can not be determined. However, with the wide application of new ionization technologies, it is now possible to efficiently ionize some intact or fragmented macromolecules