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作者在前阶段链霉菌的培养及其链霉亲和素(SA)分离纯化的基础上,对SA的生物学特性和理化性质作了测症。测得SA的分子量为72881,等电点为6.0;经SDS-PAGE电泳出现2条带,结合生物素的活性为18μg/mg。从1升链霉菌培养液中可提取纯SA23.2mg。
On the basis of Streptomyces culture and streptavidin (SA) isolation and purification in the previous stage, the biological characteristics and physico-chemical properties of SA were measured. The molecular weight of SA was 72881 and the isoelectric point was 6.0. Two bands appeared on SDS-PAGE and the activity of binding biotin was 18μg / mg. Pure SA23.2mg can be extracted from 1 liter of Streptomyces broth.