目的　探讨人乳头瘤病毒（ＨＰＶ） 和单纯疱疹病毒（ＨＳＶ） 等对宫颈癌的病因学作用。方法　应用聚合酶链反应（ＰＣＲ）核酸内切酶分型检测宫颈癌活检组织中ＨＰＶＤＮＡ和ＨＳＶＤＮＡ 基因，以正常宫颈组织作对照。结果　在宫颈癌活检组织中ＨＰＶ１６ ，１８ 型和ＨＳＶ２ 型阳性率分别为３８－９ ％和３４－６％ ，与正常妇女宫颈组织阳性率均为３－２％ 比较，差异均有非常显著意义（ Ｐ＜ ０－００１）。结论　ＨＰＶ１６，１８ 型和ＨＳＶ２ 型是诱发宫颈癌的重要病原因子 Objective To investigate the etiological effects of human papillomavirus (HPV) and herpes simplex virus (HSV) on cervical cancer. Methods Polymerase chain reaction (PCR)  endonuclease type detection of cervical cancer biopsies HPV  DNA and HSV  DNA gene to normal cervical tissue as a control. Results The positive rates of HPV16, 18 and HSV2 in cervical cancer biopsy were 38-9% and 34-6%, respectively, which were all higher than those in normal women (3-2%) Very significant (P <0-001). Conclusion HPV  16,18 and HSV  type 2 is an important pathogenic factor inducing cervical cancer