论文部分内容阅读
经研究表明顺式氯氰菊酯、甲氰菊酯、氟氰菊酯、氰戊菊酯在正辛醇——水相中的分配系数(logP)分别为6.13,5.50,5.55和4.02。离体培养的大白鼠原代肝细胞对上述杀虫剂(除氟氰菊酯外)的吸收系数(q值)分别为322.6,281.1,201.9。上述杀虫剂在离体培养的原代肝细胞中降解很快,温育30分钟后降解速率分别为90.0％(顺式氯氰菊酯)、86.2％(甲氰菊酯)、69.5％(氰戊菊酯)。同时有明显的减少离体培养的鼠肝细胞中P-450含量的作用,其中以溴氰菊酯和顺式氯氰菊酯作用较明显。在溴氰菊酯处理组中,随杀虫剂浓度的升高,这种减少P-450含量的作用明显增强。检查温育反应90分种后的细胞悬浮反应液,对照组仍维持高的细胞存活率(74.4％和91.2％),而杀虫剂处理组,除甲氰菊酯处理组仍有42.4％的存活率外,其余各杀虫剂(溴氰菊酯各浓度组和顺式氯氰菊酯处理组)均未检出存活的细胞。结果表明,试验所用的几种拟除虫菊酯类杀虫剂具有一定的细胞毒性。它们在鼠肝细胞中的降解代谢可能主要不是由于多功能氧化酶起作用。另外,杀虫剂的亲脂性与q值及在肝细胞中的降解速率三者间有一定的正相关。
The results showed that the partition coefficients (log P) of cis-cypermethrin, fenpropathrin, fenpropathrin and fenvalerate in n-octanol-water phase were 6.13, 5.50, 5.55 and 4.02, respectively. The absorption coefficients (q values) of the isolated rat primary hepatocytes cultured in vitro to the above insecticides except for cypermethrin were 322.6, 281.1 and 201.9, respectively. The above-mentioned insecticides degrade rapidly in primary hepatocytes cultured in vitro, and the degradation rates are 90.0% (cypermethrin), 86.2% (fenpropathrin), 69.5% (fenvalerate) after 30 minutes of incubation ester). At the same time, the effect of reducing P-450 content in isolated rat hepatocytes was obvious. The effect of deltamethrin and cis-cypermethrin was obvious. In the Deltamethrin treatment group, the effect of reducing the P-450 content was significantly enhanced with increasing insecticide concentrations. After 90 minutes of incubation, the cell viability of the cells in the control group remained high (74.4% and 91.2%). In the insecticide-treated group, 42.4% of the cells in the fenpropathrin-treated group Survival rate, the remaining insecticides (deltamethrin concentration groups and cypermethrin treatment group) were not detected in the survival of cells. The results showed that several pyrethroid insecticides used in the experiment had certain cytotoxicity. Their biodegradation in murine hepatocytes may not be primarily due to multifunctional oxidases. In addition, there was a certain positive correlation between the lipophilicity of insecticides and q value and the rate of degradation in hepatocytes.