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采用电子显微镜技术,研究了苹果黑星病菌在苹果叶片上发育过程。扫描电子显微镜观察结果表明,接种后12 h 分生孢子即可萌发并形成附着胞,统计结果显示其孢子萌发率在6 h和12 h分别为83% 和95%,附着胞形成率在12 h和24 h 分别为93% 和95%。透射电子显微镜观察结果表明,黑星病菌侵入以后在寄主角质层下和表皮细胞之间扩展、定殖并可形成子座。接种后12d,病菌开始从子座上产生分生孢子梗和分生孢子,分生孢子梗顶端每产生一个单生的分生孢子就形成一个环痕并延伸其长度。分生孢子梗和分生孢子主要沿叶脉形成,在叶片上呈网状扩展,此时叶片表现明显的病害症状。
Using electron microscopy, the developmental process of apple powdery mildew on apple leaves was studied. The results of scanning electron microscopy showed that the conidia germinated and formed adherent cells 12 h after inoculation. The results showed that the spore germination rate was 83% and 95% at 6 and 12 h, respectively, and the rate of adherent formation was 12 h And 24 h were 93% and 95% respectively. Transmission electron microscopy results showed that the invasion of Staphylococcus aureus in the host after the stratum corneum and epidermal cells between the expansion, colonization and the formation of sub-seat. On the 12th day after inoculation, the germs began to produce conidiophores and conidia from the submounts. Each apical conidiophore produced a solitary conidia forming a ring mark and extending its length. The conidiophores and conidia formed mainly along the veins and reticulated on the leaves. At this time, the leaves showed obvious disease symptoms.