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目的 :探讨p16基因失活对白血病发病及预后的关系。方法 :应用聚合酶链反应 (PCR)与DNA单链构象多态性分析 (SSCP)及DNA测序技术 ,甲基化检测技术。结果 :p16基因的失活形式主要以缺失和甲基化为主。p16基因缺失与急性淋巴细胞白血病的发生密切相关 ,甲基化则主要发生在髓系白血病。结论 :p16基因结构发生变化在高危白血病患者中比较多见 ,p16基因的检测对于疾病的治疗及判断预后有着重要的意义
Objective: To investigate the relationship between p16 gene inactivation and leukemia incidence and prognosis. Methods: Polymerase chain reaction (PCR) and DNA single strand conformation polymorphism (SSCP) and DNA sequencing were used to detect methylation status. Results: The inactivation of p16 gene was mainly dominated by deletion and methylation. Deletion of p16 gene is closely related to the occurrence of acute lymphoblastic leukemia, while methylation mainly occurs in myeloid leukemia. Conclusion: The change of p16 gene structure is more common in high-risk leukemia patients. The detection of p16 gene is of great significance for the treatment of disease and prognosis