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目的研究人乳腺癌细胞株（MCF－7）中增殖细胞核抗原（PCNA）的表达与细胞增殖的关系。方法对人乳腺癌细胞株MCF－7传代后培养，每天收获部分细胞，连续8天，分别作增殖细胞核抗原（PCNA）检测及溴脱氧尿核苷（BrdU）参入测定，比较PCNA阳性细胞标记指数（PCNALI）与BrdU标记指数（BrdULI）的动态变化的相关性。结果在MCF－7细胞传代培养中，PCNALI与BrdULI两个指标之间存在着显著的相关性（γ=0．92，P＜0．01）。结论PCNALI作为细胞增殖指标，为细胞增殖动力学变化提供了可靠信息。
Objective To investigate the relationship between the expression of proliferating cell nuclear antigen (PCNA) and cell proliferation in human breast cancer cell line (MCF-7). METHODS: Human breast cancer cell line MCF-7 was subcultured, and some cells were harvested daily for 8 consecutive days. Proliferating cell nuclear antigen (PCNA) detection and bromodeoxyuridine (BrdU) incorporation assay were performed. The PCNA positive cell labeling index was compared. (PCNALI) and the dynamic change of the BrdU labeling index (BrdULI). Results There was a significant correlation between PCNALI and BrdULI in the subculture of MCF-7 cells (γ=0.92, P<0.01). Conclusion PCNALI as an indicator of cell proliferation provides reliable information for cell proliferation dynamics.