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目的 探讨戊型肝炎病毒(HEV)在猪和牛群中的感染情况。方法 用抗-HEV抗体试剂盒检测猪和牛血清中的抗体;对部分血清用逆转录聚合酶链的方法(RT-PCR)检测HEV RNA,并对PCR阳性产物进行克隆测序,然后将序列进行分析。结果 281份猪血清中有235份为抗-HEV抗体阳性,阳性率为83.6％;其中56份血清中有3份为HEV RNA阳性,序列分析显示从猪中克隆的3株序列(SB 2、SH1和SJ 14)之间在ORF 2(6007-6354bp)区域的核苷酸序列的同源性为83％～86％,该3株序列在ORF 2区与1,2,3,4型的同源性分别为74％～79％、73％～77％,73％～76％和83％～93％;190份牛血清中有12份为抗-HEV抗体阳性,阳性率为6.3％。结论 HEV在猪和牛群中均有流行,但在猪群中的流行率明显高于牛群。猪感染的HEV的基因序列与人群中散发性戊型肝炎病毒的4型同源性最高。
Objective To investigate the hepatitis E virus (HEV) infection in pigs and herds. Methods Anti-HEV antibody kit was used to detect the antibody in porcine and bovine serum. HEV RNA was detected by reverse transcription polymerase chain reaction (RT-PCR) on some sera. The PCR positive products were cloned and sequenced . Results The positive rate of anti-HEV antibody in 281 swine sera was 83.6%. Three out of 56 serum samples were positive for HEV RNA. The sequence analysis showed that the three sequences (SB 2, SH1 and SJ 14) in the region of ORF 2 (6007-6354 bp) ranged from 83% to 86%. The three sequences were identical in ORF region 2 with type 1, 2, 3, 4 Homology was 74% -79%, 73% -77%, 73% -76%, and 83% -93%, respectively. Twelve of the 190 bovine serum samples were positive for anti-HEV antibody with a positive rate of 6.3%. Conclusion HEV is endemic in pigs and herds, but its prevalence in pigs is significantly higher than that in herds. The gene sequence of pig-infected HEV has the highest homology to sporadic hepatitis E virus type 4 in the population.