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为了掌握细菌种群在褐潮生消过程中的作用,于2013年4—8月采用高通量测序技术调查了秦皇岛近岸海域褐潮暴发敏感海域细菌种群的结构特征.结果表明:研究区域内细菌种群丰度和多样性在8月最高、5月最低;不同月份水体中细菌种群种类组成及其丰度均存在明显差异,其中第一优势门为变形菌门(Proteobacteria),2013年4月、5月、6月、7月和8月相对丰度分别为46.26%、41.11%、49.69%、38.48%和40.55%;第一优势科为α-变形菌(α-proteobacteria),相对丰度分别为42.20%、34.36%、44.68%、32.17%和28.79%;4月、5月和7月第一优势属为Chloroplast_norank,相对丰度分别为21.48%、16.44%和11.66%;6月第一优势属为Roseobacter_clade_DC5-80-3_lineage,相对丰度为17.85%;8月第一优势属为Candidatus_pelagibacter,相对丰度为15.55%.典范相关分析(CCA)结果显示,ρ(NO3-N)、ρ(Chla)和富营养化指数(卡尔森指数,TLI)是研究区域内影响细菌种群结构的主要因素,表明细菌种群结构特征与水体富营养化状态、藻类生物量及其特征密切相关,细菌对研究区域有害赤潮暴发的调节作用还有待进一步研究.
In order to understand the role of bacterial population in the process of browning and greening, the structural characteristics of bacterial populations in the brown sea and brownfield outbreak-sensitive waters in the coastal area of Qinhuangdao were investigated using high-throughput sequencing technology from April to August 2013. The results showed that: The abundance and diversity of bacterial population were the highest in August and the lowest in May. There were significant differences in species composition and abundance of bacterial populations in different months, of which the first dominant species was Proteobacteria, and in April 2013 , And the relative abundances in May, June, July and August were 46.26%, 41.11%, 49.69%, 38.48% and 40.55% respectively. The first dominant families were α-proteobacteria, relative abundance Respectively, which were 42.20%, 34.36%, 44.68%, 32.17% and 28.79% respectively. The first dominant species in April, May and July was Chloroplast_norank, with relative abundances of 21.48%, 16.44% and 11.66% The dominant genus was Roseobacter_clade_DC5-80-3_lineage, with a relative abundance of 17.85%. The first dominant genus in August was Candidatus pelagibacter, with a relative abundance of 15.55%. Canonical correlation analysis (CCA) showed that ρ (NO3-N), ρ ( Chla) and eutrophication index (Carlson Index, TLI) are the most important bacterial species in the study area The main structural factors, suggesting that structural features of the bacterial population is closely related to the state of eutrophication, algae biomass and its characteristics, remains to be further studied bacteria harmful red tide outbreak of the study area of regulation.