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目的建立空肠弯曲菌的快速检测方法。方法用PCR法扩增标本菌株中弯曲菌属16SrRNA片段、空肠弯曲菌M apA片段和结肠弯曲菌CeuE片段,通过电泳后对产物进行分析。结果11份标本中有7份检出弯曲菌属16SrRNA片段,其中3份样品中检测到空肠弯曲菌M apA片段,4份样品中检测到结肠弯曲菌CeuE片段。结论PCR技术可用于空肠弯曲菌的快速检测。
Objective To establish a rapid detection method of Campylobacter jejuni. Methods The Campylobacter 16S rRNA fragment, Campylobacter jejuni M apA fragment and Campylobacter coli CeuE fragment were amplified by PCR. The products were analyzed by electrophoresis. Results Among the 11 specimens, Campylobacter 16S rRNA fragments were detected in 7 samples. Campylobacter jejuni M apA fragment was detected in 3 samples, and C. Coli Campylobacter CeuE fragment was detected in 4 samples. Conclusion PCR technique can be used for the rapid detection of Campylobacter jejuni.