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目的探讨埃博拉病毒(Ebola virus)包膜蛋白GP DNA表达质粒对小鼠中和抗体的诱导作用。方法将质粒pcDNA3.1、pcDNA3.1-GP(全长GP)、pcDNA3.1-GPΔTM(去跨膜段的GP)和pcDNA3.1-GPΔMe(t去起始密码子的GP)分别经肌肉注射免疫BALB/c小鼠,ELISA法检测血清GP抗体滴度;Western blot法检测抗体的特异性;利用埃博拉假病毒感染293E细胞模型观察中和抗体对埃博拉假病毒的中和作用。结果全长的埃博拉病毒GP和去跨膜段的GP可诱导有效的免疫反应,且诱导生成的GP抗体特异性良好;GP抗血清可有效抑制埃博拉假病毒进入293E细胞。结论埃博拉病毒GP DNA表达质粒可诱导高滴度的中和抗体,并可有效中和埃博拉假病毒。
Objective To investigate the induction of mouse neutralizing antibodies by Ebola virus envelope protein GP DNA expression plasmid. Methods Plasmids pcDNA3.1, pcDNA3.1-GP (full-length GP), pcDNA3.1-GPΔTM (transmembrane GP) and pcDNA3.1-GPΔMe (t to start codon GP) The BALB / c mice were immunized by injection, the titer of serum GP was detected by ELISA, the specificity of antibody was detected by Western blot, and the neutralization effect of neutralizing antibody on Ebola virus was observed by using Ebola virus infected 293E cell model. . Results Full-length Ebola GP and anti-transmembrane GP induced an effective immune response and the induced GP antibodies showed good specificity. GP antiserum effectively inhibited the entry of Ebola virus into 293E cells. Conclusion Ebola virus GP DNA expression plasmid can induce high titers of neutralizing antibodies and effectively neutralize Ebola virus.