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为探讨外源性神经生长因子(NGF)和降钙素基因相关肽(CGRP)对局灶性脑缺血再灌注大鼠海马神经元tau蛋白过度磷酸化的影响,用线栓法制作局灶性脑缺血再灌注模型,应用免疫组织化学SABC法、Western Blotting和图像分析方法检测大鼠海马tau蛋白在Ser199/202位点磷酸化程度和总tau蛋白表达,以及NGF与CGRP对tau蛋白过度磷酸化的影响。结果显示:缺血再灌注组同侧海马tau蛋白在Ser199/202位点磷酸化水平和总tau蛋白明显升高(P<0.05);NGF组及CGRP组大鼠海马tau蛋白在Ser199/202位点磷酸化水平明显低于缺血再灌注组,总tau也下降(P<0.05);NGF与CGRP合用组海马tau蛋白磷酸化水平进一步降低,分别低于NGF组和CGRP组(P<0.05)。以上结果表明NGF及CGRP明显减轻局灶性脑缺血再灌注大鼠海马tau蛋白磷酸化程度,二者合用作用更强,降低缺血神经元tau蛋白磷酸化水平可能对缺血神经元起保护作用。
To investigate the effect of exogenous NGF and CGRP on tau hyperphosphorylation in hippocampus after focal cerebral ischemia reperfusion in rats, The expression of tau protein and phosphorylation of tau at Ser199 / 202 in rat hippocampus were detected by immunohistochemical SABC, Western Blotting and image analysis, and the effect of NGF and CGRP on tau hyperphosphorylation Effect of phosphorylation. The results showed that phosphorylation of tau at Ser199 / 202 site and total tau protein were significantly increased in ipsilateral hippocampus of ischemia-reperfusion group (P <0.05). In hippocampus of NGF group and CGRP group, tau protein in Ser199 / 202 The level of tau phosphorylation in hippocampus was also lower than that in NGF group and CGRP group (P <0.05) . The above results show that NGF and CGRP significantly reduce the level of tau phosphorylation in the hippocampus after focal cerebral ischemia / reperfusion in rats, and their combined effect is stronger. Decreasing the phosphorylation level of tau in ischemic neurons may protect the ischemic neurons effect.