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目的探讨携带急性髓细胞白血病1/821(acutemyeloidleukemia1/eighttwentyone,AML1/ETO)融合基因的儿童急性髓细胞白血病(AML)的形态学特点。方法对46例经多重逆转录聚合酶链反应(RTPCR)检测AML1/ETO融合基因阳性的儿童AML骨髓片用瑞特染液或与姬姆萨染液一起染色后,按照国内AML分型标准进行观察分析,同时,对免疫分型及细胞化学染色结果也进行了回顾分析。结果46例AML1/ETO融合基因阳性患儿中M2a、M2b、M4EO、M4、M5b分别占67.2%、19.6%、2.2%、6.6%、4.4%,其中M2b的构成比显著低于文献报道。免疫分型、细胞化学结果均支持形态学分型。结论儿童AML患者AML1/ETO融合基因阳性不能提示形态学是否可诊断为M2b;AML1/ETO融合基因对儿童M2b没有成人特异。
Objective To investigate the morphological characteristics of children with acute myeloid leukemia (AML) carrying acute myeloid leukemia 1/821 (AML1 / ETO) fusion gene. Methods 46 children with AML1 / ETO fusion gene positive AML1 / ETO fusion gene detected by multiplex reverse transcriptase polymerase chain reaction (RTPCR) were stained with Reiter stain or Giemsa stain, and then according to the national AML classification standard Observation and analysis, the same time, immunophenotyping and cytochemical staining results were also analyzed. Results The M2a, M2b, M4EO, M4 and M5b accounted for 67.2%, 19.6%, 2.2%, 6.6% and 4.4% of the 46 children with positive AML1 / ETO fusion gene respectively. The M2b ratio was significantly lower than that reported in the literature. Immunophenotyping, cytochemical results support morphological typing. Conclusions The positive AML1 / ETO fusion gene in children with AML can not indicate whether the morphology can be diagnosed as M2b. The AML1 / ETO fusion gene has no adult specificity for M2b in children.