从我省各地征集150个大豆品种,通过种子带毒情况的测定,从中选择种子带毒率最高表现花叶和皱缩症状的“陈寨大豆”,进行了种子带毒毒源的分离。该分离物,能侵染大豆、菜豆、决明。陈寨大豆种子带毒率为27.59%。桃蚜、蚕豆蚜和棉蚜均可传毒,麦长管蚜不传毒。病毒的致死温度为63—70℃;稀释限点为10~(-4)—10~(-5);体外保毒期为3—4天,可与SMV抗血清呈阳性反应,初步认为我们所鉴定的毒源分离物为大豆花叶病毒(Soybean mosaic virus)。 A total of 150 soybean cultivars were collected from all over the province. From the determination of the poisoning of the seeds, “Chenzhai Soybean”, which has the highest performance of mosaic and shrunken symptoms of seed, was selected for the separation of seed poisoning sources. The isolate, can infect soybeans, beans, Cassia. Chenzhai soybean seed poisoning rate was 27.59%. Peach aphid, silkworm aphid and cotton aphid can be transmitted poison, wheat aphid not poisoning. The lethal temperature of the virus is 63-70 ℃; the limit of dilution is 10 -4 -10 -10; in vitro protection period is 3-4 days, which is positive with SMV antiserum, The identified virulent isolate was Soybean mosaic virus.