目的:观察葶苈草颗粒对炎症性肠病(IBD)的治疗作用并探讨其作用机制。方法:选昆明种小鼠,用3%恶唑酮(OXZ)复制小鼠OXZ结肠炎模型。实验分为正常对照、模型对照组(等容蒸馏水)、葶苈草颗粒(1.95,3.9,7.8 g·kg-1)治疗组、柳氮磺胺吡啶(0.39 g·kg-1)阳性药对照组,ig每天1次,连续治疗4 d。用酶联免疫吸附(ELISA)法测定血清和结肠组织中白细胞介素-10(IL-10)、结肠组织中髓过氧化物酶(MPO)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的含量,进行疾病活动指数(DAI)评分和病理组织学观察。结果:治疗4 d后,与模型组比较,葶苈草颗粒3.9,7.8 g·kg-1治疗组,血清和结肠组织IL-10含量明显升高(P<0.05或P<0.01),结肠组织MPO,IL-1β,TNF-α和IL-6含量明显降低(P<0.05或P<0.01);葶苈草颗粒治疗组总DAI值明显减少(P<0.05或P<0.01)。结论:葶苈草颗粒对OXZ诱导的小鼠实验性肠炎具有较好的治疗作用,其可能通过调节促炎细胞因子IL-1β,TNF-α,IL-6和抑炎细胞因子IL-10之间的平衡,有效减轻实验性结肠炎炎症,从而起到治疗IBD的作用。 Objective: To observe the therapeutic effect of Souchucao granule on inflammatory bowel disease (IBD) and to explore its mechanism. Methods: Kunming mice were selected and the mouse model of OXZ colitis was replicated with 3% oxazolone (OXZ). The experimental group was divided into normal control group, model control group (isodistilled distilled water), Aconite granules (1.95,3.9,7.8 g · kg-1), sulfasalazine (0.39 g · kg -1) , ig 1 day, continuous treatment 4 d. IL-10, MPO, IL-1β, leukocyte count in serum and colon tissue were determined by enzyme linked immunosorbent assay (ELISA) (IL-6) and tumor necrosis factor-α (TNF-α) were measured. The disease activity index (DAI) score and histopathology were observed. Results: Compared with the model group, the content of IL-10 in the serum and colon tissue of the treatment group was obviously increased (P <0.05 or P <0.01) The levels of MPO, IL-1β, TNF-α and IL-6 were significantly decreased (P <0.05 or P <0.01). The DAI in the treatment group was significantly decreased (P <0.05 or P <0.01). CONCLUSION: Achnatherum splendens granules have a good therapeutic effect on OXZ-induced mouse experimental enteritis, which may be mediated through the regulation of proinflammatory cytokines IL-1β, TNF-α, IL-6 and anti-inflammatory cytokine IL-10 Between the balance, effectively reduce the inflammation of experimental colitis, which play a role in the treatment of IBD.