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目的评价防己诺林碱调控紫杉醇对耐药卵巢癌SKOV 3/ADM细胞多药耐药的作用。方法采用MTT法和细胞摄取研究防己诺林碱调控紫杉醇对SKOV 3/ADM细胞多药耐药的作用。结果细胞生长抑制试验表明,紫杉醇对SKOV 3/ADM细胞耐药明显。0.5、1和5μg·mL~(-1)浓度的防己诺林碱分别与紫杉醇联用后,紫杉醇对细胞的半数抑制浓度(IC_(50))降为(0.542±0.117)、(0.924±0.153)和(1.931±0.375)μg·mL~(-1),耐药逆转倍数If分别为16.1、9.4和4.5倍,与单用紫杉醇相比差异有统计学意义(P<0.05)。细胞摄取试验研究表明,联用防己诺林碱浓度达到0.5μg·mL~(-1)及以上时,能够显著增加紫杉醇的细胞摄取(P<0.05)。结论防己诺林碱是潜在的逆转紫杉醇对SKOV 3/ADM细胞多药耐药候选活性化合物。
OBJECTIVE To evaluate the effect of anti-ninoxicaplatin on the multidrug resistance of drug-resistant ovarian cancer cell line SKOV 3 / ADM. Methods MTT and cell uptake were used to study the effect of anti-tubulin on paclitaxel-induced multidrug resistance in SKOV 3 / ADM cells. Results The cell growth inhibition test showed that paclitaxel had obvious drug resistance to SKOV 3 / ADM cells. After treated with paclitaxel 0.5, 0.5, 1 and 5 μg · mL -1, respectively, the half inhibitory concentration (IC 50) of paclitaxel was reduced to (0.542 ± 0.117) and (0.924 ± 0.153) ) And (1.931 ± 0.375) μg · mL -1, respectively. The fold-to-multiple reverse If was 16.1, 9.4 and 4.5 times, respectively, which was significantly different from that of paclitaxel alone (P <0.05). Cell uptake test study showed that combination of methenamine concentration of 0.5μg · mL -1 and above, can significantly increase paclitaxel cell uptake (P <0.05). Conclusions Antimanine is a potentially active compound that reverses the multidrug resistance of paclitaxel in SKOV 3 / ADM cells.