壳聚糖(CTS)在PBS缓冲溶液中对异硫氰酸荧光素(FIT℃)具有明显的荧光猝灭作用,且发现荧光猝灭程度与壳聚糖的量在一定范围内呈线性关系,吐温-20(T20)对此反应有明显的增敏效果,据此建立了通过荧光猝灭测定壳聚糖含量的方法。实验结果表明:在pH6.4的PBS缓冲溶液及T20含量为0.00200%的体系中,壳聚糖浓度在0.0400—1.00mg/L范围内的回归方程为:△F=13603C—4.5948,r=0.9992。对0.0400mg/L的壳聚糖平行测定9次,检出限为0.0350mg/L,RSD为2.70%。本方法具有简便易行、干扰小的特点,可用于壳聚糖医用制剂的含量检测和相关研究。 Chitosan (CTS) has obvious fluorescence quenching effect on fluorescein isothiocyanate (FIT ° C) in PBS buffer solution, and found that the degree of fluorescence quenching is linear with the amount of chitosan within a certain range, Tween-20 (T20) has obvious sensitizing effect to this reaction, thus the method of determining the content of chitosan by fluorescence quenching was established. The experimental results show that the regression equation of the concentration of chitosan in the range of 0.0400-1.00mg / L is: △ F = 13603C-4.5948, r = 0.9992 in the system of pH6.4 PBS buffer solution and T20 content of 0.00200% . The parallel determination of 0.0400mg / L chitosan 9 times, the detection limit of 0.0350mg / L, RSD was 2.70%. The method has the characteristics of simple and convenient operation and small interference, and can be used for the content detection and the related research of the chitosan medical preparation.