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目的:探讨荧光定量PCR对浙贝母鉴别的可行性。方法:采用实时荧光定量PCR法从试样中提取DNA,进行引物扩增,并通过Cq值和扩增曲线对样品进行判断。结果:4批浙贝母的Cq值均低于30,曲线有明显的指数增长期;平贝母、川贝母和土贝母则无Cq值,曲线为一直线。结论:本方法用于鉴别浙贝母操作简单,准确率高,重现性好,可行有效。
Objective: To investigate the feasibility of fluorescence quantitative PCR for the identification of Fritillaria cirrhosa. Methods: DNA was extracted from the samples by real-time fluorescence quantitative PCR, and primers were amplified. The samples were judged by Cq value and amplification curve. Results: The results showed that the Cq values of four batches of Fritillaria cirrhosae were all less than 30, and the curve had a clear exponential growth phase. There was no Cq value for Fritillaria cirrhosa, Fritillaria cirrhosa and Fritillaria thunbergii, and the curve was a straight line. Conclusion: The method is suitable for identification of Fritillaria cirrhosae with simple operation, high accuracy, good reproducibility, and feasible and effective.